anti p atf2 Search Results


rabbit anti p atf2 monoclonal antibody  (Bioss)
93
Bioss rabbit anti p atf2 monoclonal antibody
ANKRD49 activates JNK pathway to regulate the expression of MMP-2/MMP-9 in H1299 cells. (A) The MAPK protein levels in ANKRD49-OE and ANKRD49-sh H1299 cells were assessed by Western blot. (B, C) The effects of JNK inhibitor or p38 MAPK inhibitor on the levels of MMP-2/MMP- 9 in ANKRD49-OE H1299 cells were tested by Western blot. β-Tubulin served as an internal control. (D, E) A wound healing assay was conducted to assess the effect of JNK inhibitor (SP600125) or p38 inhibitor (SB203580) on the migration of ANKRD49-OE H1299 cells; representative images were taken at 40× magnification at 0 and 24 h. (F) The levels of <t>p-ATF2</t> and p-c-Jun in ANKRD49-OE and ANKRD49-sh H1299 cells were measured by Western blot. (G, H) The levels of <t>p-ATF2</t> and p-c-Jun in ANKRD49-OE H1299 cells treated with SP600125, SB203580 or DMSO were detected by Western blot. Data are expressed as means ± standard deviation. * P <0.05, ** P <0.01, *** P <0.001 vs LV5 group.
Rabbit Anti P Atf2 Monoclonal Antibody, supplied by Bioss, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+p+atf2/ATF2+(Thr69+71)+Polyclonal+Antibody/bio_rxiv__2023__03__22__533821-136-29-50
Average 93 stars, based on 1 article reviews
rabbit anti p atf2 monoclonal antibody - by Bioz Stars, 2026-09
93/100 stars
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anti-phospho-atf2 (thr69-thr51) polyclonal antibody  (TargetMol)
N/A
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transcription factor 2  (Santa Cruz Biotechnology)
93
Santa Cruz Biotechnology transcription factor 2
ANKRD49 activates JNK pathway to regulate the expression of MMP-2/MMP-9 in H1299 cells. (A) The MAPK protein levels in ANKRD49-OE and ANKRD49-sh H1299 cells were assessed by Western blot. (B, C) The effects of JNK inhibitor or p38 MAPK inhibitor on the levels of MMP-2/MMP- 9 in ANKRD49-OE H1299 cells were tested by Western blot. β-Tubulin served as an internal control. (D, E) A wound healing assay was conducted to assess the effect of JNK inhibitor (SP600125) or p38 inhibitor (SB203580) on the migration of ANKRD49-OE H1299 cells; representative images were taken at 40× magnification at 0 and 24 h. (F) The levels of <t>p-ATF2</t> and p-c-Jun in ANKRD49-OE and ANKRD49-sh H1299 cells were measured by Western blot. (G, H) The levels of <t>p-ATF2</t> and p-c-Jun in ANKRD49-OE H1299 cells treated with SP600125, SB203580 or DMSO were detected by Western blot. Data are expressed as means ± standard deviation. * P <0.05, ** P <0.01, *** P <0.001 vs LV5 group.
Transcription Factor 2, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+p+atf2/p-ATF-2+Antibody/pm27035565-90-137-141
Average 93 stars, based on 1 article reviews
transcription factor 2 - by Bioz Stars, 2026-09
93/100 stars
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anti p atf 2 f 1 antibody  (Santa Cruz Biotechnology)
94
Santa Cruz Biotechnology anti p atf 2 f 1 antibody
ANKRD49 activates JNK pathway to regulate the expression of MMP-2/MMP-9 in H1299 cells. (A) The MAPK protein levels in ANKRD49-OE and ANKRD49-sh H1299 cells were assessed by Western blot. (B, C) The effects of JNK inhibitor or p38 MAPK inhibitor on the levels of MMP-2/MMP- 9 in ANKRD49-OE H1299 cells were tested by Western blot. β-Tubulin served as an internal control. (D, E) A wound healing assay was conducted to assess the effect of JNK inhibitor (SP600125) or p38 inhibitor (SB203580) on the migration of ANKRD49-OE H1299 cells; representative images were taken at 40× magnification at 0 and 24 h. (F) The levels of <t>p-ATF2</t> and p-c-Jun in ANKRD49-OE and ANKRD49-sh H1299 cells were measured by Western blot. (G, H) The levels of <t>p-ATF2</t> and p-c-Jun in ANKRD49-OE H1299 cells treated with SP600125, SB203580 or DMSO were detected by Western blot. Data are expressed as means ± standard deviation. * P <0.05, ** P <0.01, *** P <0.001 vs LV5 group.
Anti P Atf 2 F 1 Antibody, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+p+atf2/ATF-2/10__1097_slash_01__wcb__0000084251__20114__65-45-10-13
Average 94 stars, based on 1 article reviews
anti p atf 2 f 1 antibody - by Bioz Stars, 2026-09
94/100 stars
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anti p atf2 thr71  (Cusabio)
94
Cusabio anti p atf2 thr71
The p38 MAPK activator LX-3 rescues the malignant behavior of HPSCC cells repressed by the silencing of THBS1 (A) Western blot analysis of the extent of p38, <t>ATF2,</t> and MK2 phosphorylation, as well as total p38 expression in KD-THBS1-treated HPSCC cells. (B) Western blot analysis of the extent of p38, <t>ATF2,</t> and MK2 phosphorylation, as well as total p38 expression in HPSCC cells treated with 2 μM LX-3 after knockdown of THBS1. (C–E) HPSCC cell proliferative capacity was examined using EdU staining. Transwell assay detects changes in the migratory (D) and invasive (E) abilities of HPSCC cells. (F) Expression of E-cadherin and N-cadherin in HPSCC cells was examined using dual-labeling immunofluorescence. The results are expressed as the mean ± SD (A–F). These values are derived from three independent experiments. Statistical analysis was performed using an unpaired t test.
Anti P Atf2 Thr71, supplied by Cusabio, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+p+atf2/ATF2/pmc12924756-380-36-55
Average 94 stars, based on 1 article reviews
anti p atf2 thr71 - by Bioz Stars, 2026-09
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anti p atf2  (Cell Signaling Technology Inc)
91
Cell Signaling Technology Inc anti p atf2
The p38 MAPK activator LX-3 rescues the malignant behavior of HPSCC cells repressed by the silencing of THBS1 (A) Western blot analysis of the extent of p38, <t>ATF2,</t> and MK2 phosphorylation, as well as total p38 expression in KD-THBS1-treated HPSCC cells. (B) Western blot analysis of the extent of p38, <t>ATF2,</t> and MK2 phosphorylation, as well as total p38 expression in HPSCC cells treated with 2 μM LX-3 after knockdown of THBS1. (C–E) HPSCC cell proliferative capacity was examined using EdU staining. Transwell assay detects changes in the migratory (D) and invasive (E) abilities of HPSCC cells. (F) Expression of E-cadherin and N-cadherin in HPSCC cells was examined using dual-labeling immunofluorescence. The results are expressed as the mean ± SD (A–F). These values are derived from three independent experiments. Statistical analysis was performed using an unpaired t test.
Anti P Atf2, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+p+atf2/Phospho-ATF-2+(Thr69%2F71)%2FATF-7+(Thr51%2F53)+Antibody/ppr0229692-85-35-38
Average 91 stars, based on 1 article reviews
anti p atf2 - by Bioz Stars, 2026-09
91/100 stars
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anti p atf2 antibody  (Santa Cruz Biotechnology)
94
Santa Cruz Biotechnology anti p atf2 antibody
The p38 MAPK activator LX-3 rescues the malignant behavior of HPSCC cells repressed by the silencing of THBS1 (A) Western blot analysis of the extent of p38, <t>ATF2,</t> and MK2 phosphorylation, as well as total p38 expression in KD-THBS1-treated HPSCC cells. (B) Western blot analysis of the extent of p38, <t>ATF2,</t> and MK2 phosphorylation, as well as total p38 expression in HPSCC cells treated with 2 μM LX-3 after knockdown of THBS1. (C–E) HPSCC cell proliferative capacity was examined using EdU staining. Transwell assay detects changes in the migratory (D) and invasive (E) abilities of HPSCC cells. (F) Expression of E-cadherin and N-cadherin in HPSCC cells was examined using dual-labeling immunofluorescence. The results are expressed as the mean ± SD (A–F). These values are derived from three independent experiments. Statistical analysis was performed using an unpaired t test.
Anti P Atf2 Antibody, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+p+atf2/ATF-2+Antibody/pm18385943-93-19-21
Average 94 stars, based on 1 article reviews
anti p atf2 antibody - by Bioz Stars, 2026-09
94/100 stars
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anti p atf2  (Novus Biologicals)
91
Novus Biologicals anti p atf2
The p38 MAPK activator LX-3 rescues the malignant behavior of HPSCC cells repressed by the silencing of THBS1 (A) Western blot analysis of the extent of p38, <t>ATF2,</t> and MK2 phosphorylation, as well as total p38 expression in KD-THBS1-treated HPSCC cells. (B) Western blot analysis of the extent of p38, <t>ATF2,</t> and MK2 phosphorylation, as well as total p38 expression in HPSCC cells treated with 2 μM LX-3 after knockdown of THBS1. (C–E) HPSCC cell proliferative capacity was examined using EdU staining. Transwell assay detects changes in the migratory (D) and invasive (E) abilities of HPSCC cells. (F) Expression of E-cadherin and N-cadherin in HPSCC cells was examined using dual-labeling immunofluorescence. The results are expressed as the mean ± SD (A–F). These values are derived from three independent experiments. Statistical analysis was performed using an unpaired t test.
Anti P Atf2, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+p+atf2/ATF2+%5Bp+Thr69%5D+Antibody/pm34798593-98-35-36
Average 91 stars, based on 1 article reviews
anti p atf2 - by Bioz Stars, 2026-09
91/100 stars
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rabbit anti-phospho-atf2-t71/t53 antibody  (RayBiotech)
N/A
Phospho-ATF2-T71/T53 Polyclonal Antibody
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anti-phospho-atf2 (thr55/73), polyclonal  (Thermo Fisher)
N/A
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anti-phospho-atf-2 (s480) antibody  (St Johns Laboratory)
N/A
Rabbit polyclonal to Phospho-ATF-2 (S480). Conjugation note: Unconjugated Application note: WB, IHC-p, IF, ELISA Reactivity note: Human, Rat
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anti-phospho-atf2 (ser62-ser44) polyclonal antibody  (TargetMol)
N/A
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Image Search Results


ANKRD49 activates JNK pathway to regulate the expression of MMP-2/MMP-9 in H1299 cells. (A) The MAPK protein levels in ANKRD49-OE and ANKRD49-sh H1299 cells were assessed by Western blot. (B, C) The effects of JNK inhibitor or p38 MAPK inhibitor on the levels of MMP-2/MMP- 9 in ANKRD49-OE H1299 cells were tested by Western blot. β-Tubulin served as an internal control. (D, E) A wound healing assay was conducted to assess the effect of JNK inhibitor (SP600125) or p38 inhibitor (SB203580) on the migration of ANKRD49-OE H1299 cells; representative images were taken at 40× magnification at 0 and 24 h. (F) The levels of p-ATF2 and p-c-Jun in ANKRD49-OE and ANKRD49-sh H1299 cells were measured by Western blot. (G, H) The levels of p-ATF2 and p-c-Jun in ANKRD49-OE H1299 cells treated with SP600125, SB203580 or DMSO were detected by Western blot. Data are expressed as means ± standard deviation. * P <0.05, ** P <0.01, *** P <0.001 vs LV5 group.

Journal: bioRxiv

Article Title: ANKRD49 promotes the metastasis of NCI-H1299 and NCI-H1703 cells via activating JNK-ATF2/c-Jun-MMP-2/9 axis

doi: 10.1101/2023.03.22.533821

Figure Lengend Snippet: ANKRD49 activates JNK pathway to regulate the expression of MMP-2/MMP-9 in H1299 cells. (A) The MAPK protein levels in ANKRD49-OE and ANKRD49-sh H1299 cells were assessed by Western blot. (B, C) The effects of JNK inhibitor or p38 MAPK inhibitor on the levels of MMP-2/MMP- 9 in ANKRD49-OE H1299 cells were tested by Western blot. β-Tubulin served as an internal control. (D, E) A wound healing assay was conducted to assess the effect of JNK inhibitor (SP600125) or p38 inhibitor (SB203580) on the migration of ANKRD49-OE H1299 cells; representative images were taken at 40× magnification at 0 and 24 h. (F) The levels of p-ATF2 and p-c-Jun in ANKRD49-OE and ANKRD49-sh H1299 cells were measured by Western blot. (G, H) The levels of p-ATF2 and p-c-Jun in ANKRD49-OE H1299 cells treated with SP600125, SB203580 or DMSO were detected by Western blot. Data are expressed as means ± standard deviation. * P <0.05, ** P <0.01, *** P <0.001 vs LV5 group.

Article Snippet: Rabbit anti-ANKRD49 primary antibody (Proteintech, Cat# 25034-1-AP, RRID:AB_2879860), rabbit anti-MMP-2 monoclonal antibody (Cat# bs-4599R, RRID:AB_11083963), rabbit anti-MMP-9 monoclonal antibody (Cat# bs-0397R, RRID:AB_10853038), rabbit anti-c-Jun monoclonal antibody (Cat# bs-0670R, RRID:AB_10857880), rabbit anti-p-ATF2 monoclonal antibody (Cat# bs-3033R, RRID:AB_10883830) and Alexa Fluor ® 488-conjugated goat anti-rabbit IgG (Cat# bs-0295G-A488, RRID:AB_10893781) were obtained from Bioss Biotechnology (Beijing, China).

Techniques: Expressing, Western Blot, Control, Wound Healing Assay, Migration, Standard Deviation

ANKRD49 activates c-Jun/ATF2 transcription factor to regulate the expression of MMP- 2/MMP-9 in H1299 cells. (A) The levels of ATF2 and c-Jun in the nuclear fraction of ANKRD49-OE or LV5 H1299 cells were measured by Western blot. (B) The levels of p-ATF2 and p-c-Jun in the nuclear fraction of ANKRD49- OE or LV5 H1299 cells were measured by Western blot. (C) Co-immunoprecipitation (Co-IP) analysis was performed to assess the interaction between p-ATF2 and p-c-Jun in the nucleus of ANKRD49-OE or vector H1299 cells. (D) Representative immunofluorescence images of ANKRD49 induced nuclear co-location of p-ATF2 and p-c-Jun. Scale bar:100 μm. (E-H) Chromatin immunoprecipitating (CHIP) assay was carried out to analyze the binding of ATF2 or c-Jun with the promoter of MMP-2 or MMP-9. β-Tubulin, and PARP or Histone-H3 served as the internal control for cytosol and nuclear fractions, respectively. Data are expressed as means ± standard deviation. * P <0.05, ** P <0.01, *** P <0.001 vs LV5 group.

Journal: bioRxiv

Article Title: ANKRD49 promotes the metastasis of NCI-H1299 and NCI-H1703 cells via activating JNK-ATF2/c-Jun-MMP-2/9 axis

doi: 10.1101/2023.03.22.533821

Figure Lengend Snippet: ANKRD49 activates c-Jun/ATF2 transcription factor to regulate the expression of MMP- 2/MMP-9 in H1299 cells. (A) The levels of ATF2 and c-Jun in the nuclear fraction of ANKRD49-OE or LV5 H1299 cells were measured by Western blot. (B) The levels of p-ATF2 and p-c-Jun in the nuclear fraction of ANKRD49- OE or LV5 H1299 cells were measured by Western blot. (C) Co-immunoprecipitation (Co-IP) analysis was performed to assess the interaction between p-ATF2 and p-c-Jun in the nucleus of ANKRD49-OE or vector H1299 cells. (D) Representative immunofluorescence images of ANKRD49 induced nuclear co-location of p-ATF2 and p-c-Jun. Scale bar:100 μm. (E-H) Chromatin immunoprecipitating (CHIP) assay was carried out to analyze the binding of ATF2 or c-Jun with the promoter of MMP-2 or MMP-9. β-Tubulin, and PARP or Histone-H3 served as the internal control for cytosol and nuclear fractions, respectively. Data are expressed as means ± standard deviation. * P <0.05, ** P <0.01, *** P <0.001 vs LV5 group.

Article Snippet: Rabbit anti-ANKRD49 primary antibody (Proteintech, Cat# 25034-1-AP, RRID:AB_2879860), rabbit anti-MMP-2 monoclonal antibody (Cat# bs-4599R, RRID:AB_11083963), rabbit anti-MMP-9 monoclonal antibody (Cat# bs-0397R, RRID:AB_10853038), rabbit anti-c-Jun monoclonal antibody (Cat# bs-0670R, RRID:AB_10857880), rabbit anti-p-ATF2 monoclonal antibody (Cat# bs-3033R, RRID:AB_10883830) and Alexa Fluor ® 488-conjugated goat anti-rabbit IgG (Cat# bs-0295G-A488, RRID:AB_10893781) were obtained from Bioss Biotechnology (Beijing, China).

Techniques: Expressing, Western Blot, Immunoprecipitation, Co-Immunoprecipitation Assay, Plasmid Preparation, Immunofluorescence, Binding Assay, Control, Standard Deviation

Knockdown of ANKRD49 downregulates MMP-2/MMP-9 expression of H1703 cells. (A, B) The mRNA and protein levels of MMP-2 and MMP-9 in ANKRD49-sh H1703 cells were analyzed by RT-qPCR and Western blot. (C, D) The activities of MMP-2 and MMP-9 in ANKRD49-sh H1703 cells were detected by gelatin zymography. (E) The MAPK proteins in ANKRD49-sh H1703 cells were detected by Western blot. GAPDH served as an internal control. (F) The levels of p-ATF2 and p-c-Jun in ANKRD49-sh H1703 cells were measured by Western blot. (G, H) The levels of JNK, p-JNK, p38, p- p38, MMP-2 and MMP-9 in ANKRD49-sh H1703 cells treated with SP600125, SB203580, Anisomycin or DMSO were measured by Western blot. β-Tubulin served as an internal control. Data are expressed as means ± standard deviation. * P <0.05, ** P <0.01, *** P <0.001 vs LV3 group.

Journal: bioRxiv

Article Title: ANKRD49 promotes the metastasis of NCI-H1299 and NCI-H1703 cells via activating JNK-ATF2/c-Jun-MMP-2/9 axis

doi: 10.1101/2023.03.22.533821

Figure Lengend Snippet: Knockdown of ANKRD49 downregulates MMP-2/MMP-9 expression of H1703 cells. (A, B) The mRNA and protein levels of MMP-2 and MMP-9 in ANKRD49-sh H1703 cells were analyzed by RT-qPCR and Western blot. (C, D) The activities of MMP-2 and MMP-9 in ANKRD49-sh H1703 cells were detected by gelatin zymography. (E) The MAPK proteins in ANKRD49-sh H1703 cells were detected by Western blot. GAPDH served as an internal control. (F) The levels of p-ATF2 and p-c-Jun in ANKRD49-sh H1703 cells were measured by Western blot. (G, H) The levels of JNK, p-JNK, p38, p- p38, MMP-2 and MMP-9 in ANKRD49-sh H1703 cells treated with SP600125, SB203580, Anisomycin or DMSO were measured by Western blot. β-Tubulin served as an internal control. Data are expressed as means ± standard deviation. * P <0.05, ** P <0.01, *** P <0.001 vs LV3 group.

Article Snippet: Rabbit anti-ANKRD49 primary antibody (Proteintech, Cat# 25034-1-AP, RRID:AB_2879860), rabbit anti-MMP-2 monoclonal antibody (Cat# bs-4599R, RRID:AB_11083963), rabbit anti-MMP-9 monoclonal antibody (Cat# bs-0397R, RRID:AB_10853038), rabbit anti-c-Jun monoclonal antibody (Cat# bs-0670R, RRID:AB_10857880), rabbit anti-p-ATF2 monoclonal antibody (Cat# bs-3033R, RRID:AB_10883830) and Alexa Fluor ® 488-conjugated goat anti-rabbit IgG (Cat# bs-0295G-A488, RRID:AB_10893781) were obtained from Bioss Biotechnology (Beijing, China).

Techniques: Knockdown, Expressing, Quantitative RT-PCR, Western Blot, Zymography, Control, Standard Deviation

Overexpression of ANKRD49 promotes the migration and invasion of H1299 cells in nude mice. (A) Representative lung images of mice injected with ANKRD49-OE-H1299 or LV5-H1299 cells. White arrows manifest the metastasis nodules on the lung. (B) Statistical analysis of the number of metastasis nodules on the lung was illustrated. (C) Representative images of HE staining for lung metastases. Greenarrows indicated metastatic H1299 cells. (D) Representative images of IHC staining for NAPSA and NKX2-1. Scale bars represent 100 μm. (E, F) Representative images of IHC staining for ANKRD49, MMP-2, MMP-9, p-JNK, p-ATF2 or p-c-Jun. Scale bars represent 100 μm. (G) Quantitative analysis of IHC staining. ** P <0.01, *** P <0.001 vs LV5 group.

Journal: bioRxiv

Article Title: ANKRD49 promotes the metastasis of NCI-H1299 and NCI-H1703 cells via activating JNK-ATF2/c-Jun-MMP-2/9 axis

doi: 10.1101/2023.03.22.533821

Figure Lengend Snippet: Overexpression of ANKRD49 promotes the migration and invasion of H1299 cells in nude mice. (A) Representative lung images of mice injected with ANKRD49-OE-H1299 or LV5-H1299 cells. White arrows manifest the metastasis nodules on the lung. (B) Statistical analysis of the number of metastasis nodules on the lung was illustrated. (C) Representative images of HE staining for lung metastases. Greenarrows indicated metastatic H1299 cells. (D) Representative images of IHC staining for NAPSA and NKX2-1. Scale bars represent 100 μm. (E, F) Representative images of IHC staining for ANKRD49, MMP-2, MMP-9, p-JNK, p-ATF2 or p-c-Jun. Scale bars represent 100 μm. (G) Quantitative analysis of IHC staining. ** P <0.01, *** P <0.001 vs LV5 group.

Article Snippet: Rabbit anti-ANKRD49 primary antibody (Proteintech, Cat# 25034-1-AP, RRID:AB_2879860), rabbit anti-MMP-2 monoclonal antibody (Cat# bs-4599R, RRID:AB_11083963), rabbit anti-MMP-9 monoclonal antibody (Cat# bs-0397R, RRID:AB_10853038), rabbit anti-c-Jun monoclonal antibody (Cat# bs-0670R, RRID:AB_10857880), rabbit anti-p-ATF2 monoclonal antibody (Cat# bs-3033R, RRID:AB_10883830) and Alexa Fluor ® 488-conjugated goat anti-rabbit IgG (Cat# bs-0295G-A488, RRID:AB_10893781) were obtained from Bioss Biotechnology (Beijing, China).

Techniques: Over Expression, Migration, Injection, Staining, Immunohistochemistry

Knockdown of ANKRD49 declines the migration and invasion of H1703 cells in nude mice. (A) Representative lung images of mice injected with ANKRD49-sh or LV3 H1703 cells. White arrows manifest the metastasis nodules on the lung. (B) Statistical analysis of the number of metastasis nodules on the lung was illustrated. (C) Representative images of HE staining for lung metastases. Green arrows indicated metastatic H1703 cells. (D) Representative images of IHC staining for p63. Scale bars represent 100 μm. (E, F) Representative images of IHC staining for ANKRD49, MMP-2, MMP-9, p-JNK, p-ATF2 or p-c-Jun. Scale bars represent 100 μm. (G) Quantitative analysis of IHC staining. ** P <0.01, *** P <0.001 vs LV3 group.

Journal: bioRxiv

Article Title: ANKRD49 promotes the metastasis of NCI-H1299 and NCI-H1703 cells via activating JNK-ATF2/c-Jun-MMP-2/9 axis

doi: 10.1101/2023.03.22.533821

Figure Lengend Snippet: Knockdown of ANKRD49 declines the migration and invasion of H1703 cells in nude mice. (A) Representative lung images of mice injected with ANKRD49-sh or LV3 H1703 cells. White arrows manifest the metastasis nodules on the lung. (B) Statistical analysis of the number of metastasis nodules on the lung was illustrated. (C) Representative images of HE staining for lung metastases. Green arrows indicated metastatic H1703 cells. (D) Representative images of IHC staining for p63. Scale bars represent 100 μm. (E, F) Representative images of IHC staining for ANKRD49, MMP-2, MMP-9, p-JNK, p-ATF2 or p-c-Jun. Scale bars represent 100 μm. (G) Quantitative analysis of IHC staining. ** P <0.01, *** P <0.001 vs LV3 group.

Article Snippet: Rabbit anti-ANKRD49 primary antibody (Proteintech, Cat# 25034-1-AP, RRID:AB_2879860), rabbit anti-MMP-2 monoclonal antibody (Cat# bs-4599R, RRID:AB_11083963), rabbit anti-MMP-9 monoclonal antibody (Cat# bs-0397R, RRID:AB_10853038), rabbit anti-c-Jun monoclonal antibody (Cat# bs-0670R, RRID:AB_10857880), rabbit anti-p-ATF2 monoclonal antibody (Cat# bs-3033R, RRID:AB_10883830) and Alexa Fluor ® 488-conjugated goat anti-rabbit IgG (Cat# bs-0295G-A488, RRID:AB_10893781) were obtained from Bioss Biotechnology (Beijing, China).

Techniques: Knockdown, Migration, Injection, Staining, Immunohistochemistry

Correlation analysis between H-score of ANKRD49, MMP-2, MMP-9, p-JNK, p-ATF2 or p-c-Jun and lung metastasis in mice injected with ANKRD49-OE H1299 cells. (A) Correlation analysis between H-score of ANKRD49 and lung metastasis in mice injected with ANKRD49-OE H1299 cells. (B) Correlation analysis between H-score of MMP-2 and lung metastasis in mice injected with ANKRD49-OE H1299 cells. (C) Correlation analysis between H-score of MMP-9 and lung metastasis in mice injected with ANKRD49-OE H1299 cells. (D) Correlation analysis between H-score of p-JNK and lung metastasis in mice injected with ANKRD49-OE H1299 cells. (E) Correlation analysis between H-score of p-ATF2 and lung metastasis in mice injected with ANKRD49-OE H1299 cells. (F) Correlation analysis between H-score of p-c-Jun and lung metastasis in mice injected with ANKRD49-OE H1299 cells.

Journal: bioRxiv

Article Title: ANKRD49 promotes the metastasis of NCI-H1299 and NCI-H1703 cells via activating JNK-ATF2/c-Jun-MMP-2/9 axis

doi: 10.1101/2023.03.22.533821

Figure Lengend Snippet: Correlation analysis between H-score of ANKRD49, MMP-2, MMP-9, p-JNK, p-ATF2 or p-c-Jun and lung metastasis in mice injected with ANKRD49-OE H1299 cells. (A) Correlation analysis between H-score of ANKRD49 and lung metastasis in mice injected with ANKRD49-OE H1299 cells. (B) Correlation analysis between H-score of MMP-2 and lung metastasis in mice injected with ANKRD49-OE H1299 cells. (C) Correlation analysis between H-score of MMP-9 and lung metastasis in mice injected with ANKRD49-OE H1299 cells. (D) Correlation analysis between H-score of p-JNK and lung metastasis in mice injected with ANKRD49-OE H1299 cells. (E) Correlation analysis between H-score of p-ATF2 and lung metastasis in mice injected with ANKRD49-OE H1299 cells. (F) Correlation analysis between H-score of p-c-Jun and lung metastasis in mice injected with ANKRD49-OE H1299 cells.

Article Snippet: Rabbit anti-ANKRD49 primary antibody (Proteintech, Cat# 25034-1-AP, RRID:AB_2879860), rabbit anti-MMP-2 monoclonal antibody (Cat# bs-4599R, RRID:AB_11083963), rabbit anti-MMP-9 monoclonal antibody (Cat# bs-0397R, RRID:AB_10853038), rabbit anti-c-Jun monoclonal antibody (Cat# bs-0670R, RRID:AB_10857880), rabbit anti-p-ATF2 monoclonal antibody (Cat# bs-3033R, RRID:AB_10883830) and Alexa Fluor ® 488-conjugated goat anti-rabbit IgG (Cat# bs-0295G-A488, RRID:AB_10893781) were obtained from Bioss Biotechnology (Beijing, China).

Techniques: Injection

Correlation analysis between H-score of MMP-2, MMP-9, p-JNK, p-ATF2 or p-c-Jun and ANKRD49 in lung tissues of mice injected with ANKRD49-OE H1299 cells. (A) Correlation analysis between H-score of MMP-2 and ANKRD49 in lung tissues of mice injected with ANKRD49-OE H1299 cells. (B) Correlation analysis between H-score of MMP-9 and ANKRD49 in lung tissues of mice injected with ANKRD49-OE H1299 cells. (C) Correlation analysis between H- score of p-JNK and ANKRD49 in lung tissues of mice injected with ANKRD49-OE H1299 cells. (D) Correlation analysis between H-score of p-ATF2 and ANKRD49 in lung tissues of mice injected with ANKRD49-OE H1299 cells. (E) Correlation analysis between H-score of p-c-Jun and ANKRD49 in lung tissues of mice injected with ANKRD49-OE H1299 cells.

Journal: bioRxiv

Article Title: ANKRD49 promotes the metastasis of NCI-H1299 and NCI-H1703 cells via activating JNK-ATF2/c-Jun-MMP-2/9 axis

doi: 10.1101/2023.03.22.533821

Figure Lengend Snippet: Correlation analysis between H-score of MMP-2, MMP-9, p-JNK, p-ATF2 or p-c-Jun and ANKRD49 in lung tissues of mice injected with ANKRD49-OE H1299 cells. (A) Correlation analysis between H-score of MMP-2 and ANKRD49 in lung tissues of mice injected with ANKRD49-OE H1299 cells. (B) Correlation analysis between H-score of MMP-9 and ANKRD49 in lung tissues of mice injected with ANKRD49-OE H1299 cells. (C) Correlation analysis between H- score of p-JNK and ANKRD49 in lung tissues of mice injected with ANKRD49-OE H1299 cells. (D) Correlation analysis between H-score of p-ATF2 and ANKRD49 in lung tissues of mice injected with ANKRD49-OE H1299 cells. (E) Correlation analysis between H-score of p-c-Jun and ANKRD49 in lung tissues of mice injected with ANKRD49-OE H1299 cells.

Article Snippet: Rabbit anti-ANKRD49 primary antibody (Proteintech, Cat# 25034-1-AP, RRID:AB_2879860), rabbit anti-MMP-2 monoclonal antibody (Cat# bs-4599R, RRID:AB_11083963), rabbit anti-MMP-9 monoclonal antibody (Cat# bs-0397R, RRID:AB_10853038), rabbit anti-c-Jun monoclonal antibody (Cat# bs-0670R, RRID:AB_10857880), rabbit anti-p-ATF2 monoclonal antibody (Cat# bs-3033R, RRID:AB_10883830) and Alexa Fluor ® 488-conjugated goat anti-rabbit IgG (Cat# bs-0295G-A488, RRID:AB_10893781) were obtained from Bioss Biotechnology (Beijing, China).

Techniques: Injection

Illustration the binding site of ATF2/c-Jun on MMP-2 or MMP-9 promoter region. (A) illustration the binding site of ATF2/c-Jun on MMP-2 promoter region. (B) illustration the binding site of ATF2/c-Jun on MMP-9 promoter region.

Journal: bioRxiv

Article Title: ANKRD49 promotes the metastasis of NCI-H1299 and NCI-H1703 cells via activating JNK-ATF2/c-Jun-MMP-2/9 axis

doi: 10.1101/2023.03.22.533821

Figure Lengend Snippet: Illustration the binding site of ATF2/c-Jun on MMP-2 or MMP-9 promoter region. (A) illustration the binding site of ATF2/c-Jun on MMP-2 promoter region. (B) illustration the binding site of ATF2/c-Jun on MMP-9 promoter region.

Article Snippet: Rabbit anti-ANKRD49 primary antibody (Proteintech, Cat# 25034-1-AP, RRID:AB_2879860), rabbit anti-MMP-2 monoclonal antibody (Cat# bs-4599R, RRID:AB_11083963), rabbit anti-MMP-9 monoclonal antibody (Cat# bs-0397R, RRID:AB_10853038), rabbit anti-c-Jun monoclonal antibody (Cat# bs-0670R, RRID:AB_10857880), rabbit anti-p-ATF2 monoclonal antibody (Cat# bs-3033R, RRID:AB_10883830) and Alexa Fluor ® 488-conjugated goat anti-rabbit IgG (Cat# bs-0295G-A488, RRID:AB_10893781) were obtained from Bioss Biotechnology (Beijing, China).

Techniques: Binding Assay

The p38 MAPK activator LX-3 rescues the malignant behavior of HPSCC cells repressed by the silencing of THBS1 (A) Western blot analysis of the extent of p38, ATF2, and MK2 phosphorylation, as well as total p38 expression in KD-THBS1-treated HPSCC cells. (B) Western blot analysis of the extent of p38, ATF2, and MK2 phosphorylation, as well as total p38 expression in HPSCC cells treated with 2 μM LX-3 after knockdown of THBS1. (C–E) HPSCC cell proliferative capacity was examined using EdU staining. Transwell assay detects changes in the migratory (D) and invasive (E) abilities of HPSCC cells. (F) Expression of E-cadherin and N-cadherin in HPSCC cells was examined using dual-labeling immunofluorescence. The results are expressed as the mean ± SD (A–F). These values are derived from three independent experiments. Statistical analysis was performed using an unpaired t test.

Journal: iScience

Article Title: THBS1 upregulation by KLF7 in hypopharyngeal squamous cell carcinoma contributes to lung metastasis through the p38 MAPK signaling

doi: 10.1016/j.isci.2026.114816

Figure Lengend Snippet: The p38 MAPK activator LX-3 rescues the malignant behavior of HPSCC cells repressed by the silencing of THBS1 (A) Western blot analysis of the extent of p38, ATF2, and MK2 phosphorylation, as well as total p38 expression in KD-THBS1-treated HPSCC cells. (B) Western blot analysis of the extent of p38, ATF2, and MK2 phosphorylation, as well as total p38 expression in HPSCC cells treated with 2 μM LX-3 after knockdown of THBS1. (C–E) HPSCC cell proliferative capacity was examined using EdU staining. Transwell assay detects changes in the migratory (D) and invasive (E) abilities of HPSCC cells. (F) Expression of E-cadherin and N-cadherin in HPSCC cells was examined using dual-labeling immunofluorescence. The results are expressed as the mean ± SD (A–F). These values are derived from three independent experiments. Statistical analysis was performed using an unpaired t test.

Article Snippet: The primary antibodies used were as follows: anti-THBS1 (1:250, A2125, BioAb), anti-E-cadherin (1:1000, 3195, Cell Signaling Technologies), anti-N-cadherin (1:100, PA5-19486m Invitrogen), anti-Phospho-p38 MAPK (Thr180/Tyr182) (1:1000, 4511, Cell Signaling Technologies), anti-total p38 (1:1000, 9212, Cell Signaling Technologies), anti- p -ATF2 (Thr71) (1:1000, 44-295G, Invitrogen), anti-p-MK2 (Thr334) (1:1000, 3007, Cell Signaling Technologies), anti-KLF7 (1:1000, CSB- PA126129 , Cusabio), anti-Slug (1:1000, 12129-1-AP, ProteinTech Group), anti-Vimentin (1:20000, 10366-1-AP, ProteinTech Group), anti-HDAC6 (1:1000, 12834-1-AP, ProteinTech Group), and anti-GAPDH (1:20000, AC001, BioAb).

Techniques: Western Blot, Phospho-proteomics, Expressing, Knockdown, Staining, Transwell Assay, Labeling, Immunofluorescence, Derivative Assay

Reactivation of THBS1 rescues the HPSCC cell malignant biological behavior curtailed by knockdown of KLF7 (A) Western blot analysis of protein expression of KLF7 and THBS1 in HPSCC cells treated with knockdown of KLF7 combined with the overexpression of THBS1. (B–D) HPSCC cell proliferative capacity was examined using EdU staining. Transwell assay detects changes in the migratory (C) and invasive (D) abilities of HPSCC cells. (E) Expression of E-cadherin and N-cadherin in HPSCC cells was examined using dual-labeling immunofluorescence. (F) Western blot analysis of the extent of p38, ATF2, and MK2 phosphorylation, as well as total p38 expression in HPSCC cells. The results are expressed as the mean ± SD (A–F). These values are derived from three independent experiments. Statistical analysis was performed using a one-way ANOVA followed by Tukey’s multiple comparison test.

Journal: iScience

Article Title: THBS1 upregulation by KLF7 in hypopharyngeal squamous cell carcinoma contributes to lung metastasis through the p38 MAPK signaling

doi: 10.1016/j.isci.2026.114816

Figure Lengend Snippet: Reactivation of THBS1 rescues the HPSCC cell malignant biological behavior curtailed by knockdown of KLF7 (A) Western blot analysis of protein expression of KLF7 and THBS1 in HPSCC cells treated with knockdown of KLF7 combined with the overexpression of THBS1. (B–D) HPSCC cell proliferative capacity was examined using EdU staining. Transwell assay detects changes in the migratory (C) and invasive (D) abilities of HPSCC cells. (E) Expression of E-cadherin and N-cadherin in HPSCC cells was examined using dual-labeling immunofluorescence. (F) Western blot analysis of the extent of p38, ATF2, and MK2 phosphorylation, as well as total p38 expression in HPSCC cells. The results are expressed as the mean ± SD (A–F). These values are derived from three independent experiments. Statistical analysis was performed using a one-way ANOVA followed by Tukey’s multiple comparison test.

Article Snippet: The primary antibodies used were as follows: anti-THBS1 (1:250, A2125, BioAb), anti-E-cadherin (1:1000, 3195, Cell Signaling Technologies), anti-N-cadherin (1:100, PA5-19486m Invitrogen), anti-Phospho-p38 MAPK (Thr180/Tyr182) (1:1000, 4511, Cell Signaling Technologies), anti-total p38 (1:1000, 9212, Cell Signaling Technologies), anti- p -ATF2 (Thr71) (1:1000, 44-295G, Invitrogen), anti-p-MK2 (Thr334) (1:1000, 3007, Cell Signaling Technologies), anti-KLF7 (1:1000, CSB- PA126129 , Cusabio), anti-Slug (1:1000, 12129-1-AP, ProteinTech Group), anti-Vimentin (1:20000, 10366-1-AP, ProteinTech Group), anti-HDAC6 (1:1000, 12834-1-AP, ProteinTech Group), and anti-GAPDH (1:20000, AC001, BioAb).

Techniques: Knockdown, Western Blot, Expressing, Over Expression, Staining, Transwell Assay, Labeling, Immunofluorescence, Phospho-proteomics, Derivative Assay, Comparison

Activation of the THBS1/p38 MAPK axis by KLF7 promotes the lung metastasis of HPSCC cells (A) Lung metastasis in nude mice injected with FaDu cells in the tail vein was assessed by HE staining. (B–D) Quantitative results of lung metastatic nodules. IHC scores (C) and protein expression (D) of Slug and Vimentin within lung metastases in nude mice. (E) RT-qPCR detection of mRNA expression levels of KLF7 and THBS1 in lung metastases. (F) IHC scores of KLF7 and THBS1 within lung metastases in nude mice. (G) Western blot analysis of the extent of p38, ATF2, and MK2 phosphorylation, as well as total p38 expression in lung metastases. The results are expressed as the mean ± SD (A–G). These values are derived from five mice. Statistical analysis was performed using a one-way ANOVA followed by Tukey’s multiple comparison test.

Journal: iScience

Article Title: THBS1 upregulation by KLF7 in hypopharyngeal squamous cell carcinoma contributes to lung metastasis through the p38 MAPK signaling

doi: 10.1016/j.isci.2026.114816

Figure Lengend Snippet: Activation of the THBS1/p38 MAPK axis by KLF7 promotes the lung metastasis of HPSCC cells (A) Lung metastasis in nude mice injected with FaDu cells in the tail vein was assessed by HE staining. (B–D) Quantitative results of lung metastatic nodules. IHC scores (C) and protein expression (D) of Slug and Vimentin within lung metastases in nude mice. (E) RT-qPCR detection of mRNA expression levels of KLF7 and THBS1 in lung metastases. (F) IHC scores of KLF7 and THBS1 within lung metastases in nude mice. (G) Western blot analysis of the extent of p38, ATF2, and MK2 phosphorylation, as well as total p38 expression in lung metastases. The results are expressed as the mean ± SD (A–G). These values are derived from five mice. Statistical analysis was performed using a one-way ANOVA followed by Tukey’s multiple comparison test.

Article Snippet: The primary antibodies used were as follows: anti-THBS1 (1:250, A2125, BioAb), anti-E-cadherin (1:1000, 3195, Cell Signaling Technologies), anti-N-cadherin (1:100, PA5-19486m Invitrogen), anti-Phospho-p38 MAPK (Thr180/Tyr182) (1:1000, 4511, Cell Signaling Technologies), anti-total p38 (1:1000, 9212, Cell Signaling Technologies), anti- p -ATF2 (Thr71) (1:1000, 44-295G, Invitrogen), anti-p-MK2 (Thr334) (1:1000, 3007, Cell Signaling Technologies), anti-KLF7 (1:1000, CSB- PA126129 , Cusabio), anti-Slug (1:1000, 12129-1-AP, ProteinTech Group), anti-Vimentin (1:20000, 10366-1-AP, ProteinTech Group), anti-HDAC6 (1:1000, 12834-1-AP, ProteinTech Group), and anti-GAPDH (1:20000, AC001, BioAb).

Techniques: Activation Assay, Injection, Staining, Expressing, Quantitative RT-PCR, Western Blot, Phospho-proteomics, Derivative Assay, Comparison